shrna against p63 (Addgene inc)
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shrna against p63
Shrna Against P63, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+against+p63/shp63alpha+pLKO%2E1+puro+(Plasmid+%2319120)/pm26390052-51-19-25
Average 93 stars, based on 9 article reviews
Shrna Against P63, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+against+p63/shp63alpha+pLKO%2E1+puro+(Plasmid+%2319120)/pm26390052-51-19-25
Average 93 stars, based on 9 article reviews
shrna against p63 - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture."
Article Title: Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture.
Journal: Laboratory investigation; a journal of technical methods and pathology
doi: 10.1038/labinvest.2015.114
Figure Legend Snippet: Figure 1 Basal cell reactivity, hyperplasia and upregulation of EMT markers adjacent to fibroblastic foci. Epithelium adjacent to fibroblastic foci shows increased reactivity and mesenchymal phenotype. Sparse staining of CK14 is seen in control samples, whereas CK14 is strongly expressed in epithelium overlying fibroblastic foci. P63, a restricted transcription factor for basal cells is expressed in epithelial cells located both basally and in the top layer indicating formation of metaplasia in IPF samples. E-cadherin is abundant in epithelium both in control and IPF samples. In addition, E-cadherin is present in cells within the foci. N-cadherin is expressed in lower basal epithelial cells in IPF samples whereas control samples are negative. Vimentin is expressed in both epithelium and mesenchyme in IPF samples. Bar 100 μm.
Techniques Used: Staining, Control
Figure Legend Snippet: Figure 5 Knockdown (KD) of p63 abrogates UG-induced EMT of VA10 cells. p63 KD cells are unable to undergo UG-induced EMT. The expression of the mesenchymal markers N-cadherin and Thy-1 is abrogated in treated KD cells, whereas scrambled control cells show a division into two cellular sub-populations much like the mother cell line VA10. The expression of Vimentin can still be detected in treated KD cells, although to a lesser extent than in treated scrambled cells. Bars 100 μm.
Techniques Used: Knockdown, Expressing, Control
Related Articles
Plasmid Preparation:Article Title: deltaNp63 Has a Role in Maintaining Epithelial Integrity in Airway Epithelium Article Snippet: Primary cells were isolated from patient material with enzyme digestion and cultured in chemically defined bronchial cell growth medium as previously described Isolated cells were maintained on collagen coated flasks (VitroCol, Advanced BioMatrix) and cultured in chemically defined bronchial epithelial cell medium (BEGM, Life Technologies/Sigma) as previously described . .. For production of lentivirus containing scrambled hairpin (pLKO.1 shSCR) (Addgene plasmid 17920) or Article Title: Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture. Article Snippet: .. Lentiviral transduction was performed as previously described.28 In short, plasmids containing scrambled hairpin (pLKO.1 shSCR; Addgene plasmid 17920)29 or Article Title: deltaNp63 has a role in maintaining epithelial integrity in airway epithelium. Article Snippet: Primary cells were isolated from patient material with enzyme digestion and cultured in chemically defined bronchial cell growth medium as previously described [29] Isolated cells were maintained on collagen coated flasks (VitroCol, Advanced BioMatrix) and cultured in chemically defined bronchial epithelial cell medium (BEGM, Life Technologies/Sigma) as previously described [30]. .. Production of Lentivirus and Cell Transduction For production of lentivirus containing scrambled hairpin (pLKO.1 shSCR) (Addgene plasmid 17920) [31] or shRNA:Article Title: deltaNp63 Has a Role in Maintaining Epithelial Integrity in Airway Epithelium Article Snippet: Primary cells were isolated from patient material with enzyme digestion and cultured in chemically defined bronchial cell growth medium as previously described Isolated cells were maintained on collagen coated flasks (VitroCol, Advanced BioMatrix) and cultured in chemically defined bronchial epithelial cell medium (BEGM, Life Technologies/Sigma) as previously described . .. For production of lentivirus containing scrambled hairpin (pLKO.1 shSCR) (Addgene plasmid 17920) or Article Title: Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture. Article Snippet: .. Lentiviral transduction was performed as previously described.28 In short, plasmids containing scrambled hairpin (pLKO.1 shSCR; Addgene plasmid 17920)29 or Article Title: deltaNp63 has a role in maintaining epithelial integrity in airway epithelium. Article Snippet: Primary cells were isolated from patient material with enzyme digestion and cultured in chemically defined bronchial cell growth medium as previously described [29] Isolated cells were maintained on collagen coated flasks (VitroCol, Advanced BioMatrix) and cultured in chemically defined bronchial epithelial cell medium (BEGM, Life Technologies/Sigma) as previously described [30]. .. Production of Lentivirus and Cell Transduction For production of lentivirus containing scrambled hairpin (pLKO.1 shSCR) (Addgene plasmid 17920) [31] or Transduction:Article Title: Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture. Article Snippet: .. Lentiviral transduction was performed as previously described.28 In short, plasmids containing scrambled hairpin (pLKO.1 shSCR; Addgene plasmid 17920)29 or Article Title: deltaNp63 has a role in maintaining epithelial integrity in airway epithelium. Article Snippet: Primary cells were isolated from patient material with enzyme digestion and cultured in chemically defined bronchial cell growth medium as previously described [29] Isolated cells were maintained on collagen coated flasks (VitroCol, Advanced BioMatrix) and cultured in chemically defined bronchial epithelial cell medium (BEGM, Life Technologies/Sigma) as previously described [30]. .. Production of Lentivirus and Cell Transduction For production of lentivirus containing scrambled hairpin (pLKO.1 shSCR) (Addgene plasmid 17920) [31] or |